Equipment in FACS Core Facility
BD FACSAria™ Cell Sorter
BD FACSAria™ cell sorter, a high-speed sorter that provides high-performance sorting and analysis, is the product of numerous technological advances. This instrument comes with 488-nm, 633-nm and 407-nm lasers and can detect up to 13 colors, for a total 15 parameters. The 70 and 100-micron nozzle tip sizes can accommodate most cell types. Two and four-way sort collection devices can accommodate a variety of tube sizes (15mL, 12×75-mm, and 1mL tubes). Automatic Cell Deposition Unit (ACDU) allows for sorting into 6-, 24-, 48-, 96- and 384- well plates or onto glass slides.
| BD FACSAria™ Configuration | |||
| Laser | Wavelength (nm) | Min. Power (mW) | Commonly Used Fluorochromes |
| Coherent® Sapphire™ Solid State |
488nm (blue) | 13 | FITC, PE, PE-TxRed,PE-Cy7, PerCP, PerCPCy5.5, PI |
| JDS Uniphase™ HeNe Air Cooled |
633nm (red) | 11 | APC, APC-Cy7 |
| Point Source Violet Solid State| (optional) |
407nm (violet) | 10 | Alexa Fluor®, Cascade blue Pacific blue, DAPI, Hoeschste |
| BD FACSAria™ Detector Array | |||
| Excitation Laser | BP Filter | PMT | Intended Dye |
| 488nm (blue laser) |
488/10 (483-493nm) 780/60 (750-810nm) 695/40 (675-715nm) 610/20 (600-620nm) 576/26 (563-589nm) 530/30 (515-545nm) 488/10 (483-493nm) |
FSC Diode A B C D E F |
FSConly PE-Cy7 PE-Texas Red PerCP-Cy5.5 or P PE or PI FITC SSC only |
| 633nm (red laser) |
780/60 (750-810nm) 660/20 (650-670nm) |
A B |
APC-Cy7 APC |
| 407nm (violet laser) |
530/30 (515-545nm) 450/40 (430-470nm) |
A B |
Alexa Fluor 405, DAPI Pacific blue, Hoechst |
| BD FACSAria™ is a trademark of BD Biosciences | |||
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