Equipment in FACS Core Facility

BD FACSAria equipment setup

BD FACSAria™ Cell Sorter

BD FACSAria™ cell sorter, a high-speed sorter that provides high-performance sorting and analysis, is the product of numerous technological advances. This instrument comes with 488-nm, 633-nm and 407-nm lasers and can detect up to 13 colors, for a total 15 parameters. The 70 and 100-micron nozzle tip sizes can accommodate most cell types. Two and four-way sort collection devices can accommodate a variety of tube sizes (15mL, 12×75-mm, and 1mL tubes). Automatic Cell Deposition Unit (ACDU) allows for sorting into 6-, 24-, 48-, 96- and 384- well plates or onto glass slides.

BD FACSAria™ Configuration
Laser Wavelength (nm) Min. Power (mW) Commonly Used Fluorochromes
Coherent®
Sapphire™
Solid State
488nm (blue) 13 FITC, PE, PE-TxRed,PE-Cy7,
PerCP, PerCPCy5.5, PI
JDS Uniphase™
HeNe Air Cooled
633nm (red) 11 APC, APC-Cy7
Point Source
Violet Solid State|
(optional)
407nm (violet) 10 Alexa Fluor®, Cascade blue
Pacific blue, DAPI, Hoeschste
 
BD FACSAria™ Detector Array
Excitation Laser  BP Filter   PMT  Intended Dye
488nm
(blue laser)
488/10 (483-493nm)
780/60 (750-810nm)
695/40 (675-715nm)
610/20 (600-620nm)
576/26 (563-589nm)
530/30 (515-545nm)
488/10 (483-493nm)
FSC Diode
A
B
C
D
E
F
FSConly
PE-Cy7
PE-Texas Red
PerCP-Cy5.5 or P
PE or PI
FITC
SSC only
633nm
(red laser)
780/60 (750-810nm)
660/20 (650-670nm)
A
B
APC-Cy7
APC
407nm
(violet laser)
 530/30 (515-545nm) 
450/40 (430-470nm) 
A
B
Alexa Fluor 405, DAPI
Pacific blue, Hoechst
       
BD FACSAria™ is a trademark of BD Biosciences
       

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