Estrogen Fractions

This is a urine test that measures the amount of estriol (E²), which is the major hormone present in a pregnant female. This is measured during pregnancy around 28-30 weeks of the pregnancy. If the level is decreasing, it usually indicates that the fetus is at risk for complications and may need early delivery.


No preparation or fasting is necessary unless directed by caregiver to collect a 24-hour urine sample.


Estradiol Serum Urine μg/24 hours

  • Child less than 10 years oldless than 15pg/ml0-6

  • Adult male10-50 pg/ml0-6

  • Adult female

  • Follicular phase20-350pg/ml0-13

  • Midcycle peak150-750 pg/ml4-14

  • Luteal phase30-450 pg/ml4-10

  • Postmenopauseless than or equal to 20pg/ml0-4


  • Male or child

  • less than 10 years oldN/A1-11

  • Female, adult

  • Follicular phaseN/A0-14

  • Ovulatory phaseN/A13-54

  • Luteal phase N/A8-60

  • PostmenopausalN/A0-11

  • Female, pregnant

  • 1st trimesterless than 38ng/ml0-800

  • 2nd trimester38-140 ng/ml800-12,000

  • 3rd trimester31-460 ng/ml5000-12,000

Total estrogen

  • Male or child

  • less than 10 years oldN/A4-25

  • Female, non-pregnantN/A4-60

  • Female, pregnant

  • 1st trimesterN/A0-800

  • 2nd trimesterN/A800-5000

  • 3rd trimesterN/A5000-50,000

Results that are 40% below the average of two previous results demand immediate evaluation of baby's well-being.

Ranges for normal findings may vary among different laboratories and hospitals. You should always check with your doctor after having lab work or other tests done to discuss the meaning of your test results and whether your values are considered within normal limits.


Your caregiver will go over the test results with you and discuss the importance and meaning of your results, as well as treatment options and the need for additional tests if necessary.


It is your responsibility to obtain your test results. Ask the lab or department performing the test when and how you will get your results.